FastQCFastQC Report
Sun 14 Apr 2019
SRR6575327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622257
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21270.3418201804077736No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13480.2166307490313488No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12990.20875618916299854No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9390.15090227992613983No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7170.1152257025634103No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6530.1049405631435243No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6360.10220857298511708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2450.059.4783861
GTATCAA11250.058.4977381
ATCAACG13300.049.4732173
TCAACGC13550.048.9072884
CAACGCA13850.047.8479275
AACGCAG14500.045.703026
TATCAAC16250.040.787712
ACGCAGA17150.038.6410337
CGCAGAG18650.035.281178
TCTATAC801.157871E-535.249673
GTACATG23150.034.9253731
GGTCTAT550.002714040534.1869931
ACATGGG24100.033.3482323
TACATGG23850.033.3090252
TTAAGAC2000.032.8996933
TAGGTAT4050.032.4935235
CTATAGA1457.421477E-1032.4186971
GTCTTAG4650.032.348981
TTAGGTA4250.032.0702864
CTTAGGT4600.031.6736153
Produced by FastQC (version 0.11.2)