FastQCFastQC Report
Sun 14 Apr 2019
SRR6575327_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575327_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622257
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23380.37572899943271026No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13380.2150236959969916No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12440.19991739747403403No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8220.13209975942416075No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8060.12952847456918926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.058.9824641
GGTATCA2850.054.538121
TCAACGC12700.049.9594734
ATCAACG12800.049.5691683
CAACGCA13450.047.5230675
AACGCAG13600.047.3444986
GTACTAG405.6149013E-447.1011051
TATCAAC16050.040.1174552
ACGCAGA17700.036.3776937
CGCAGAG18200.035.1200688
TTAGGTA3550.031.7739144
GCAGAGT20350.031.6405459
GTCTTAG4050.031.4007341
TAGGTAT3500.030.8855
AGGTATA3350.030.864966
GGTAATC2000.030.5492958
GTATAGT4650.030.3218788
CTTAGGT3650.029.6157553
TACATGG22600.029.5302932
GTACATG22650.029.529171
Produced by FastQC (version 0.11.2)