FastQCFastQC Report
Sun 14 Apr 2019
SRR6575335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792618
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24240.305821972248927No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17170.2166238970096566No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16780.2117034939908001No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10740.13550032928850972No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10070.1270473292304742No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9660.12187459785167636No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8860.11178146345402198No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8050.10156216487639697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.051.9116861
GTATCAA15650.048.666621
TCAACGC16750.044.0532884
ATCAACG16800.043.922183
CAACGCA17300.042.9244275
AACGCAG18050.041.140866
TATCAAC19500.038.334782
ACGCAGA23000.032.0822877
GTACATG26050.031.5835421
TTAGGTA5550.031.333024
TACATGG26300.031.1045552
CGCAGAG24200.030.491438
GTCTTAG6900.029.980171
ACATGGG27750.029.3006083
TAGGTAT5900.028.677685
CTTAGGT5800.028.3617843
AGGTATA5900.027.8810776
GGTAATC2550.027.646788
GCAGAGT27100.027.2285089
ACCAGAT13250.026.25044894
Produced by FastQC (version 0.11.2)