FastQCFastQC Report
Sun 14 Apr 2019
SRR6575336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705395
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22970.32563315589138003No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17930.25418382608325835No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15160.21491504759744542No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10390.14729336045761593No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9450.13396749339022818No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8450.1197910390632199No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8400.11908221634686948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3500.057.7461241
GTATCAA12950.053.3543661
TCAACGC13500.050.470024
ATCAACG13900.049.35573
CAACGCA14250.047.8137055
AACGCAG14600.046.6674846
TATCAAC16000.042.8929672
ACGCAGA18500.036.8294757
CGCAGAG19550.034.8514258
GTACATG23600.034.6545031
GCAGAGT21050.032.5888639
TACATGG24550.032.5499762
GTCTTAG6100.031.5919511
ACATGGG26150.030.1881753
TTAGGTA5250.029.5361524
TAGGTAT5250.029.5361525
CTTAGGT5250.029.5361523
AGGTATA5150.029.1972566
GGTAATC2101.8189894E-1229.0886348
GGTATAG5600.026.8510487
Produced by FastQC (version 0.11.2)