FastQCFastQC Report
Sun 14 Apr 2019
SRR6575337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218139
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5180.23746326883317517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.16365711770935046No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3070.14073595276406328No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3070.14073595276406328No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA2550.11689794122096461No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2540.11643951792205887No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2340.107271051943944No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2300.10543735874832103No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2250.1031452422537923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6350.048.9148181
TTAGGTA857.8835E-944.2327084
TAGGTAT751.4742909E-743.86415
AGGTATA802.4474866E-741.1225936
CTTAGGT853.9378574E-738.703623
GTGATCG651.4102242E-436.151738
GTCTTAG1204.120011E-935.2964861
CTCTTAG550.002694729234.22692
TTAACCT550.002712984834.1798174
ATCAACG9150.033.8996543
TCTTAGG1404.984031E-1033.6157042
CAACGCA9300.033.3528865
TCAACGC9300.033.3528864
GTACTTG1151.11713234E-732.738771
GGTATCA3100.031.88071
AACGCAG9650.031.6561766
GTATAAG600.004126943631.3746551
TCGGATC300.00415936931.33150122-23
GGTATAG1052.0561802E-631.3315017
ATGGGAG1701.2187229E-1030.4099865