FastQCFastQC Report
Sun 14 Apr 2019
SRR6575337_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575337_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218139
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5040.23104534264849477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.20033098162180996No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2960.1356932964761001No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2700.12377429070455077No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA2700.12377429070455077No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2320.10635420534613252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.10497893544941528No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2250.1031452422537923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT504.5494926E-756.3944976
CCTTTAT405.61817E-447.081781
TAAGTAT652.776369E-643.3803835
ATATAGT652.776369E-643.3803834
GTATCAA5950.040.3558121
TAGTATA757.4208874E-637.5963337
GTACATG13350.035.97261
TACATGG13200.035.958612
CTTAAGT801.1551272E-535.246563
GTATAGT1204.1782187E-935.246568
TATTAGC550.002713503534.1784822
TATAGTG550.002713503534.1784825
ACATGGG13650.034.0845873
CATGGGG7700.032.3474964
GGTACCT2050.032.094438
TCAACGC7550.031.7452474
CCTATAT600.004118450531.3878541
GGGTACC1807.2759576E-1231.3302767
TCTTAAG902.5860949E-531.3302762
CCGTCGT753.2687804E-431.3302769