FastQCFastQC Report
Sun 14 Apr 2019
SRR6575344_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575344_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712033
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23190.3256871521404205No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15310.21501812415997573No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14730.20687243428324248No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8940.12555597844481928No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7530.10575352546862295No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7340.10308510981934826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.10139979467243794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13750.059.630781
GGTATCA3450.050.5366631
TCAACGC16550.047.4222224
ATCAACG16700.047.2810173
CAACGCA17000.045.890485
TATCAAC18050.044.2655332
AACGCAG17900.043.845696
GTCTTAG5950.039.598341
TTAGGTA5250.037.5970234
AGGTATA5250.037.5970236
TAGGTAT5300.037.2423325
ACGCAGA22900.034.067177
CTTAGGT5600.033.5711253
TCTTAGG6850.032.934012
CGCAGAG24050.032.438188
ACCTAAG8000.032.3963931
GGTATAG6200.031.8361877
TATAGTA6600.031.330859
GTATAGT6700.030.8632288
CTAAGAC9100.029.9557743