FastQCFastQC Report
Sun 14 Apr 2019
SRR6575345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641595
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21040.3279327301490816No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16120.25124884077961956No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14490.22584340588689125No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8670.13513197577911298No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7730.1204809887857605No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6900.10754447899375774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.10442724771857635No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6490.1011541548796359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT200.002164962570.4801256
GGTATCA3550.062.2514721
GTACCTA250.005226298756.4236761
GTACTAG352.9304496E-453.7368351
GTATCAA14050.053.54561
TCAACGC15350.047.7520034
ATCAACG15400.047.596973
CAACGCA16100.045.5275345
AACGCAG16650.044.023626
TATCAAC17450.042.3042832
ACGCAGA20300.036.1080447
CGCAGAG22050.033.0292368
GTACATG23300.031.0774171
GCAGAGT23450.031.0573439
TACATGG23400.030.3417932
ACATGGG24500.028.7673993
GTCTTAG6100.028.5201661
TAGGTAT4900.027.8084855
AGGTATA4950.027.527596
AGAGTAC19600.026.72970810-11
Produced by FastQC (version 0.11.2)