FastQCFastQC Report
Sun 14 Apr 2019
SRR6575370_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575370_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270088
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6330.2343680578181926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.18734634637599598No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3960.14661887977251858No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3700.1369923876662421No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3620.13403039009508014No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3330.12329314889961791No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3100.11477740588252718No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2900.1073724119546222No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2730.10107816711590296No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2710.10033766772311248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA450.001012743641.776389
CATATAG704.5355846E-640.3965571
GTATCAA8250.039.4172441
TAGCCCG500.001700002237.598745
TATGCAC801.1556393E-535.2488175
GGGTACC2700.034.813657
GGTACCT2750.032.4716388
CGGTTAT600.00415607931.33228394
GGACTAT600.00415607931.3322836
CATGGGG5950.030.8056894
TCAACGC10300.030.571794
ATCAACG10350.030.4241033
GTACATG14300.030.3209571
CAACGCA10450.030.1329655
GGTATCA4700.030.0825421
TACATGG14750.028.687632
ACATGGG14650.028.5519453
AACGCAG10900.028.4577646
CATGGGT4250.027.6461354
GTACCTG3450.027.2454649