FastQCFastQC Report
Sun 14 Apr 2019
SRR6575374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618092
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26900.43521029231894276No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17720.2866887130071251No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16360.26468551607204105No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10490.1697158351831119No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9810.15871423671556986No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9610.1554784724604104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.13298991088705242No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8070.13056308769568284No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA7940.1284598409298292No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7420.1200468538664147No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7210.11664930139849732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15850.058.4328461
CAACGCA16950.052.1275945
ATCAACG17300.050.8013273
TCAACGC17550.050.345454
AACGCAG17900.049.886176
TATCAAC18350.048.678552
ACGCAGA21400.041.0683637
GGTATCA3550.039.7294921
CGCAGAG22750.038.4247558
GCAGAGT23300.037.719449
GTACTAG1401.2732926E-1136.938971
ACCTAAG8500.034.2920041
ATATCGT550.00271660334.1803786
AGGTATA5550.033.8724446
TTAGGTA5750.033.511634
CTTAGGT5750.033.511633
TAGGTAT5950.032.385195
GTCTTAG7300.032.2008441
GTACATG24850.031.5944041
CCTAAGA9700.031.0190372
Produced by FastQC (version 0.11.2)