FastQCFastQC Report
Sun 14 Apr 2019
SRR6575374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618092
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28340.4585077949560906No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16510.2671123392634106No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15250.24672702445590625No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9980.16146463633245536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.13962322761012924No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8160.13201918161050458No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8050.1302395112701669No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA7890.12765089986603936No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7050.11406068999436976No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6410.10370624437785962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15700.058.7951971
ATCAACG18400.048.7889673
GGTATCA3300.048.5233081
CAACGCA18600.048.011665
TCAACGC19200.046.5112954
TATCAAC19650.045.4461562
AACGCAG19850.044.988266
ACGCAGA21900.040.5591287
AAGGGTA1900.039.57975
CGCAGAG23650.037.5579268
GCAGAGT24600.036.1075179
GTACATG25550.033.1792831
TACATGG26300.031.9891992
ACATGGG26650.031.216353
AGGGTAA2300.030.6527546
AGAGTAC22400.030.00260710-11
GGTAATC2250.029.2426348
ACCTAAG9050.029.1423681
CATGGGG16550.028.9673164
AGGTATA5550.028.7933396