FastQCFastQC Report
Sun 14 Apr 2019
SRR6575375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556827
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26120.46908644875338307No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17230.30943183430401183No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15630.2806975954829777No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11100.19934378182092463No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9070.16288721631673753No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9040.16234844933884313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.1411569482083304No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA7660.13756516835570115No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA7470.13415297749570335No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6770.12158174801150089No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA6230.11188394240940186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.054.5419921
GGTATCA4250.053.109511
ATCAACG17700.047.2514953
CAACGCA17950.046.5933955
TCAACGC18550.045.086334
AACGCAG19050.044.149616
TATCAAC19500.043.6440352
GTACTAG1101.7844286E-938.4742161
ACGCAGA22050.037.929777
CGCAGAG22950.036.442338
AAGGGTA2350.035.9893045
GCAGAGT24000.034.8479779
GTGCAAG3400.030.4273221
GTGTAAT1701.2187229E-1030.4273221
ATAGACG953.7564496E-529.675398
CTTAGGT5100.029.4814363
GTACATG23150.028.844121
TTAGGTA5400.028.7136884
ACCAGAT12300.028.26802394
ACCTAAG9150.028.2658181