FastQCFastQC Report
Sun 14 Apr 2019
SRR6575375_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575375_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556827
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26540.47662918644390445No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15990.2871627992177103No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14670.26345705219035714No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9160.1645035172504207No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8890.15965461444937115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.14187530417885627No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7610.13666722339254384No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA7110.12768777376097065No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6720.12068380304834357No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6480.11637366722518844No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT5900.10595750565256354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14450.056.056791
ATCAACG16300.049.2991183
CAACGCA16700.047.8369065
TCAACGC17000.046.9927254
TATCAAC17650.046.335432
AACGCAG17650.045.2621156
AAGGGTA2500.041.3535965
GGTATCA3350.040.7681351
ACGCAGA20550.038.874767
CGCAGAG22000.036.552428
AGGGTAA2850.036.2750856
GCAGAGT22900.034.7051549
GTACATG23700.034.3767971
TACATGG24300.033.2683142
ACATGGG23850.033.1017953
GTACTAG1001.387547E-632.9659581
CTTAGGT4500.031.3284843
AGGTATA4850.030.0365896
TTAGGTA4750.029.6796154
TAGGTAT4700.028.9955145
Produced by FastQC (version 0.11.2)