FastQCFastQC Report
Sun 14 Apr 2019
SRR6575376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757538
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28540.37674677705936865No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17480.2307475004554227No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16880.22282710570294822No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12330.16276411216335024No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11050.14586727002473804No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10750.1419070726485008No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8740.11537375022771135No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8580.11326164496038482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.1095654607425634No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8150.1075853620544448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.055.3140831
GTATCAA16150.051.2383081
ATCAACG17800.045.9422073
TCAACGC17800.045.9422074
CAACGCA18850.043.6324165
AACGCAG19700.042.2269446
TATCAAC21600.038.3101232
GTCTTAG6500.036.16691
CTTAGGT5400.035.683933
AGGTATA5500.035.0351336
GTACATG29250.033.595031
CATATAG1405.11136E-1033.570253
ACGCAGA24950.033.3415157
CGCAGAG25450.032.6864748
TAGGTAT5900.032.659875
GGTATAG6050.032.6269537
TACATGG29750.032.556292
AAGGGTA3900.032.537325
TTAGGTA5950.032.3854184
AGGGTAA3650.032.190656