FastQCFastQC Report
Sun 14 Apr 2019
SRR6575376_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575376_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757538
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28790.38004694153956636No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16670.22005496753958217No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15740.20777835567324676No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10840.143095131861372No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10400.13728684237622404No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9750.12870641472771002No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7940.10481322389107872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.10164506599008895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA156.9020863E-493.997294
GGTATCA3650.063.2611121
GTATCAA15800.054.2810061
TCAACGC17700.044.8744164
ATCAACG18000.044.387613
CAACGCA18400.043.675215
AACGCAG19350.042.016696
TATCAAC21300.037.7313082
CTTAGGT5900.035.8464283
GTACATG32550.035.0346681
TTAGGTA6050.034.9576724
TACATGG33000.034.1808362
GGTAATC3600.033.9412278
TAGGTAT6250.033.8367925
AGGTATA6200.033.3516776
GTCTTAG7700.033.0473481
ACATGGG33850.033.0448383
CGCAGAG24850.032.5281038
GGTATAG6400.032.3094377
ACGCAGA24900.032.2740487
Produced by FastQC (version 0.11.2)