FastQCFastQC Report
Sun 14 Apr 2019
SRR6575377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684365
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26920.3933573458607615No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17100.2498666647183886No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15450.22575672338591252No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11450.16730838076172802No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10520.15371914110160512No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10330.15094284482695638No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8480.12391048636327107No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8180.11952686066645724No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7880.11514323496964339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.11382814726059924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14800.052.727411
GGTATCA3500.048.353121
ATCAACG16700.046.7079963
TCAACGC16900.046.155244
CAACGCA17250.045.218755
AACGCAG17600.044.3195156
TATCAAC19350.040.5748832
ACGCAGA22400.034.8224757
CGCAGAG23050.034.0443578
GTACATG28850.032.2633481
TACATGG28900.032.0472072
GCAGAGT24950.031.8261459
ACATGGG28900.031.5430373
GTCTTAG6250.029.3342271
ATAGTAC650.006159253428.9165343
AGAGTAC21650.027.99623910-11
AGGTATA5100.027.6408066
CATGGGA12300.027.1239454
TAGGTAT5200.027.1092515
CTTAGGT5150.026.4600333
Produced by FastQC (version 0.11.2)