FastQCFastQC Report
Sun 14 Apr 2019
SRR6575377_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575377_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684365
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28350.4142526283489074No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17180.2510356315708723No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15410.22517223995967062No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10550.15415750367128653No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10240.14962775711791224No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9430.1377919677365149No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7300.10666822528913665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.10593762100633433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3700.055.9984631
GTATCAA13900.055.8977471
TCAACGC16800.044.7555054
ATCAACG17100.043.6955033
CAACGCA17400.043.2122085
AACGCAG18650.040.5679246
TATCAAC18750.040.1038632
ACGCAGA22650.032.988667
AGGTATA5450.032.7659576
TAGGTAT5650.032.4378365
GTCTTAG7250.031.8260861
CGCAGAG23750.031.4814688
TTAGGTA5800.030.78874
GCAGAGT25800.029.5268259
CTTAGGT6050.029.516443
GGTATAG6100.029.27457
AAGGGTA2750.029.0503885
ACCAGAT12650.028.99728294
AGGGTAA2600.028.918946
CTAAGAC11900.028.8278083