FastQCFastQC Report
Sun 14 Apr 2019
SRR6575393_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575393_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451814
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9740.21557543590946718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.20207430491308373No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6380.14120855042119101No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6030.1334619998494956No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5110.11309963834675331No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5110.11309963834675331No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4630.10247579756271386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13550.054.6916581
ATCAACG16400.043.8411063
TCAACGC16650.043.1828354
CAACGCA17050.042.7209855
AACGCAG17350.041.9822926
GGTATCA5550.036.5709461
ACGCAGA20350.035.793267
TATCAAC20450.035.618232
GTACATG26150.034.656941
CGCAGAG21150.034.4393778
ACATGGG25900.033.929373
TACATGG26650.033.5035132
CATGGGT5900.033.4527054
ATGGGTA4250.032.065875
GCAGAGT23950.030.2168469
GGTACCT3750.028.8224248
CATGGGA9950.027.8652444
GTAATAC856.815503E-427.642993
GTTATAC700.00883678826.8531913
CATGGGG12600.025.7343084
Produced by FastQC (version 0.11.2)