FastQCFastQC Report
Sun 14 Apr 2019
SRR6575404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417547
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.21722105535424752No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8880.21267066940967122No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5900.14130145827894824No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5070.12142345652106229No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5030.12046548053273046No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4830.1156756005910712No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4220.10106646676901043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.10106646676901043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.050.5305671
GCCTTAC500.001688472237.6556551
TCAACGC18150.037.5452424
ATCAACG18200.037.4420973
CAACGCA18850.036.150995
AACGCAG19250.035.39986
TATCAAC20750.033.067272
TAGGACA1309.165888E-932.535894
GGTATCA6400.031.6248681
ACGCAGA21700.031.403057
CGCAGAG21800.031.2589998
CATGGGT5700.030.5063614
GTACATG24250.027.7564891
GCAGAGT24850.027.6115029
TACATGG24750.027.5331782
ACATGGG24800.026.530163
CATGGGG10650.025.594224
GTGTAAC950.001292006724.7734581
AACCGCT1151.3758954E-424.5198027
GAACCGC2352.0008883E-1023.9981046
Produced by FastQC (version 0.11.2)