FastQCFastQC Report
Sun 14 Apr 2019
SRR6575405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761446
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA62900.8260598913120563No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC39870.5236090280860364No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC37210.48867549373166314No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC23550.3092799752050704No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC22500.29549042217044935No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19040.25005056169446027No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT17450.22916923852774854No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15290.2008021579993854No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA14270.187406592194325No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13520.17755691145531002No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13360.17545564623098683No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12890.16928317963453746No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG12700.16678792718065363No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12470.16376735842068907No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12190.16009014427812346No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12160.15969615704856285No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA11340.1489271727739065No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10960.1439366678661389No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG10660.1399967955705329No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT10640.1397341374174925No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT10530.13828951757577032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10410.1367135686575279No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG10060.13211705097932092No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG9650.12673255884199272No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT9600.12607591345939173No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT9150.12016610501598274No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG9020.11845882702122014No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA8890.11675154902645755No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA8450.11097306965956878No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA8370.10992243704740717No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA8250.10834648812916477No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8220.10795250089960418No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA7980.10480060306311938No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT7860.10322465414487698No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG7790.10230535060923558No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA7780.10217402153271538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4050.058.1903951
GTATCAA22250.054.2308351
TCAACGC26000.046.2841034
ATCAACG25800.046.2784963
TATCAAC26500.044.878662
CAACGCA27100.044.0584955
AACGCAG27400.043.5761036
CTTAGGT12150.037.9153483
AGGTATA12350.037.3013346
TTAGGTA12700.036.643484
TAGGTAT12750.036.4997755
GTCTTAG13150.036.201951
ACGCAGA33150.036.0105557
GGTATAG12850.035.8428547
CGCAGAG34500.034.7376758
TCTTAGG13500.034.4720152
GTATAGT13700.033.9620828
GCAGAGT35600.033.4002849
GTACATG41450.031.9534781
TATAGTA14850.031.332029
Produced by FastQC (version 0.11.2)