FastQCFastQC Report
Sun 14 Apr 2019
SRR6575426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89856
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1930.21478810541310542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.17472400284900283No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1300.14467592592592593No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1190.13243411680911682No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1020.11351495726495728No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT930.10349893162393163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG156.852704E-494.098611
TATATGG255.502831E-575.2788852
TATACGG250.00519875456.4591642
CCGGGCT250.00522173556.3963289
CGCAAGA352.9078167E-453.7706342
TAGTTCC405.650478E-446.9969494
GTATCAA3100.045.5315861
ACGGACC450.001009290741.775068
TTATTGT450.001009290741.775064
CGGACCA450.001009290741.775069
CGTCGTA250.001698752537.59755388-89
CGATCAG250.001698752537.59755378-79
AAGTCGG250.001698752537.59755360-61
AACGCAG3700.035.5652546
GCGCAAG550.002689881734.2176741
CAACGCA3850.034.1795925
AGACGGA550.002704624634.1795926
GACGGAC550.002704624634.1795927
TCAACGC3850.034.1795924
CCGTCGT352.1785365E-433.56924488-89