FastQCFastQC Report
Sun 14 Apr 2019
SRR6575429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937430
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38410.40973726038210856No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24390.26017942673052924No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24260.25879265651835337No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16470.17569311841950866No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14270.15222469944422518No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13630.1453975230150518No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11630.12406259667388497No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10650.11360848276671325No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9860.10518118686195237No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9840.1049678375985407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5050.055.8522641
GTATCAA18900.054.2220081
ATCAACG22850.043.6051643
TCAACGC23000.043.3207784
CAACGCA24100.041.1484645
TATCAAC24200.040.7929232
AACGCAG25100.039.5090876
TTACGCA550.002717048434.1810654
AGGTATA8150.032.8704386
ACGCAGA30650.032.354917
CTTAGGT8350.032.0831223
ACCTAAG13900.031.7902551
TAGGTAT8450.031.703445
CGCAGAG31650.031.3326428
GTCTTAG9550.031.0111661
GGTATAG9100.030.4718517
GTACATG37550.030.1708831
ACCAGAT15450.030.11583994
TTAGGTA9100.029.955384
TACATGG38050.029.774422