FastQCFastQC Report
Sun 14 Apr 2019
SRR6575437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71648
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.3433452434122376No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1300.18144260830728004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.1465497990174185No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1000.13957123715944617No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA850.11863555158552926No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA830.11584412684234034No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT800.11165698972755694No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA800.11165698972755694No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA740.10328271549799017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAC156.8755826E-494.09
CCCTAAT200.002154682370.499991
ACCTGTA200.002154682370.499992
GGAGGGT250.005216067756.3999988
ATGGGAG2550.053.450985
ATGGGGC558.694551E-751.2727285
ATGGGAC1151.8189894E-1244.956525
TGGGAGA1500.043.866676
GTATCAA5500.043.5818181
CATGGGC1006.85759E-1042.34
GTACATG10250.041.726831
TACATGG10250.041.726832
ACATGGG10450.040.9282263
CATGGGA6900.040.1884084
TGGGATG801.1435704E-535.2499966
AACGCAG6850.034.9927026
TGGGACT959.287269E-734.631586
GGGAGAC550.002700106234.1818167
TGGGGCT550.002700106234.1818166
CCTCTAG550.002700106234.1818161