FastQCFastQC Report
Sun 14 Apr 2019
SRR6575437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71648
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.3433452434122376No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1510.2107525681107637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.1800468959356856No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1010.14096694953104066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.13538410004466278No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG890.1242184010719071No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.12282268870031264No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA860.12003126395712371No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA830.11584412684234034No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG830.11584412684234034No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA790.11026127735596247No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC760.1060741402411791No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT730.10188700312639572No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG720.10049129075480126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTC200.00215506370.496863
TAGTAGT200.00215506370.496864
TATAGTA250.00521698856.3974882
TACAGTA250.00521698856.3974887
ATGGGAG2350.049.9977725
GGGAATG450.001007811741.7759177
TGGGAGT1051.1077645E-940.283926
GTACATG11100.038.6919331
TACATGG11150.038.3570372
ACATGGG11200.038.1858023
ATGGGAC906.0850834E-736.553935
CATGGGA7200.035.901184
CATGGGT1107.528797E-834.1802944
CATGGGC1554.0017767E-1133.3533554
TGGGAGA1001.3873159E-632.8985336
CAGTATA600.004136115331.3319369
TATAATG600.004136115331.3319362
CATGGGG2750.030.7622644
GTATCAA4850.030.1662941
ATGGGGC953.7050304E-529.6828865
Produced by FastQC (version 0.11.2)