FastQCFastQC Report
Sun 14 Apr 2019
SRR6575448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68856
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.34274427791332635No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1320.1917044266294876No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1020.14813523875914952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.14813523875914952No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1010.14668293249680492No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA980.14232601370977113No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA950.13796909492273732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.11473219472522364No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT740.10747066341350063No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG720.10456605088881143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG405.648863E-446.984023
CATGGGT1201.8189894E-1243.0686874
CTGGGTA250.001699666637.5872268-69
GTATCAA6300.035.8233681
GGGTACC801.1455972E-535.2380187
CATGGGC851.735084E-533.165194
TACATGG9850.032.936592
ATGGGTA1001.3894896E-632.8888175
ACATGGG10050.032.725193
GTACATG10000.032.4425351
ATGGGAG2250.031.3226835
AACGCAG7350.030.6834456
CATGGGA6950.029.7452814
ATCAACG7600.029.674123
GGTACCT804.7443266E-429.3650138
TGGGTAC804.7443266E-429.3650136
ATCCAGG650.00612478128.9132466
TCAACGC7800.028.9132464
GGGATGG650.00612478128.9132467
ATGGGAT1802.2919266E-1028.7124585