FastQCFastQC Report
Sun 14 Apr 2019
SRR6575448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68856
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.37033809689787384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.19315673289183224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1320.1917044266294876No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1310.19025212036714303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1150.16701522016962936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.15103985128383873No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA980.14232601370977113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.1408737074474265No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG950.13796909492273732No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA810.11763680724991285No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT790.11473219472522364No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA760.11037527593818984No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG700.10166143836412223No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG690.10020913210177762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCA200.002155649770.4891054
TGGGACC200.002155649770.4891056
ATGGGCT407.961611E-658.740925
ATTAAGG250.00521840856.3912853
GGGTACC558.6988985E-751.2648057
GGTACCT601.5768455E-647.0268948
ATGGGTA601.5847745E-646.9927375
ATGGGAG2350.043.9932025
GTATCAA5550.043.2453271
ATGGGAC1006.87578E-1042.2934655
GTACATG10100.041.9356271
TACATGG10250.041.2919042
ACATGGG10250.041.2619173
TGGGAGG802.4168367E-741.1186456
TGGGTAC704.5768284E-640.2794886
CATGGGA6950.038.5408064
AACGCAG6300.038.041746
ATGGGGG757.3500923E-637.5941935
CATGGGC1152.6993803E-936.7769244
GGGAGGT651.4010117E-436.1482627
Produced by FastQC (version 0.11.2)