FastQCFastQC Report
Fri 12 Oct 2018
SRR6575459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224856
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5750.25571921585370194No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3670.1632155690753193No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3180.14142384459387342No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2820.12541359803607643No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2620.1165190166150781No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.11474010033087843No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2530.11251645497562886No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2350.10451133169673037No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2350.10451133169673037No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2330.10362187355463053No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2310.1027324154125307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.040.7415241
GTATATC608.802553E-539.1658363
GGGCTAT550.002712667534.181096
GTATTCA600.00414984431.3396361
TAGACCT600.00415436531.3326685
GTACATG15050.030.923161
ATCAACG9650.030.6832833
CATGGGG6200.030.3219364
ACATGGG15600.030.1275633
ATGGGGA3800.029.683585
TACATGG15700.029.6429022
AACGCAG10000.029.609376
TCAACGC10050.029.462064
ATGGGCG804.7693972E-429.3743745
GGACTGT804.7693972E-429.3743746
CAACGCA10150.029.1717915
GAATAGA650.006137753428.9288941
GTATAAT1606.85086E-826.4428161
GTATAAG1257.973982E-626.3252931
ACGCAGA11500.025.7472767
Produced by FastQC (version 0.11.2)