FastQCFastQC Report
Fri 12 Oct 2018
SRR6575459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224856
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5420.2410431565090547No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3750.16677340164371865No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3450.15343152951222114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.138310741096524No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3010.13386345038602482No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2830.12585832710712636No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2780.12363468175187677No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2610.11607428754402818No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2430.10806916426512969No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2410.10717970612302986No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2280.10139822819938095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC555.1969044E-542.8277851
GTATCAC450.001000573441.876061
TGCGTAT450.001011601341.7829294
ATAGGGG608.7950684E-539.1714863
GTACATG13850.038.436781
GTATCAA7500.037.6884541
ACATGGG13900.037.536993
TACATGG14050.037.4708022
GGGTACC2300.036.7871327
CATGGGT3700.035.5719454
GGTACCT2400.035.2543378
ATGGGTA2550.035.023925
TATAGGG959.396754E-734.635842
TAGGTAT550.002710747334.1860245
ATATGCC550.002710747334.1860243
CATGGGG5300.032.815364
CTACACG753.264874E-431.337194
TAACGCG300.00416033631.33021776-77
AACGCAG8800.030.9810856
TGGGTAC2800.030.2180026
Produced by FastQC (version 0.11.2)