FastQCFastQC Report
Sun 14 Apr 2019
SRR6575470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208014
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5470.2629630697933793No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3800.18268001192227448No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3360.161527589489169No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3130.1504706413991366No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2810.13508706144778718No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2510.12066495524339707No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2400.11537684963512072No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2300.11056948090032402No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2210.10624284903900699No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2190.10528137529204765No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2170.1043199015450883No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2140.10287769092464931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAG405.653225E-447.021411
GTATCAA8100.037.7332271
ATAGTAC600.004158633831.3253
AGTGACC753.271497E-431.3249978
GTACATG14450.031.2391381
TACATGG14500.031.1314162
ACATGGG14500.030.4608633
AACGCAG9900.028.9518936
TCAACGC10450.027.8777524
CAACGCA10600.027.4832575
CATGGGG5350.027.2264024
GTAGCCT700.00883114226.853
ACGCAGA10700.026.7872667
ATCAACG10900.026.7268353
CATGGGC2650.026.59674
GGGCAGT1606.8766894E-826.430477
CGCAGAG11150.025.7061658
GTATTAG1101.01127494E-425.6480431
TATAGTA1101.01127494E-425.6480432
CATGGGT3150.025.3583354
Produced by FastQC (version 0.11.2)