FastQCFastQC Report
Sun 14 Apr 2019
SRR6575470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208014
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5380.25863643793206226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3980.1913332756449085No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3330.16008537886872998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.15575874700741296No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3040.14614400953781956No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2720.13076042958647013No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2650.12739527147211246No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2590.12451085023123443No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2430.11681906025555971No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2400.11537684963512072No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2140.10287769092464931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7150.042.7814371
GGTACCT2550.036.8739668
CATGGGT3700.035.561294
AACGCAG8450.034.4791226
GGGTACC2600.034.340097
ATAGAGT550.00271426634.175796
ATGGGTA2750.034.1757855
ATCAACG8700.033.4883423
TCAACGC8750.033.296984
CAACGCA9050.032.1932145
TAATAGA753.2697784E-431.3278054
GTACATG11700.030.9708371
ACATGGG11850.030.5346953
TACATGG11850.030.138142
ACGCAGA9700.029.5514877
CGCAGAG10050.029.0038558
GTATCGT650.00614804228.9179745
TATCGTG650.00614804228.9179746
TGGGTAC3200.027.9013276
ATCCTAG856.7470165E-427.6821061