FastQCFastQC Report
Sun 14 Apr 2019
SRR6575481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478980
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5100.10647626205687086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.10313583030606706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.045.2145041
ATAGACG555.279432E-542.7259458
ATCAACG11650.039.938673
CAACGCA12100.038.064935
TCAACGC12550.037.449034
AACGCAG12950.035.9293866
TATCAAC14100.032.9989742
ACGCAGA14450.032.199697
GTACATG18850.031.1824131
TACATGG19300.030.4394682
CGCAGAG15350.030.311768
TAGACGA804.7776388E-429.3740869
ACATGGG20000.028.90413
CCCCGTA459.512443E-426.11302624-25
GCAGAGT17750.025.948499
CATGGGT4900.025.8971544
GTATAAC1201.8297661E-423.511541
CATGGGA11300.022.0435644
ACGATCA1303.1565898E-421.6916359
GTACTTG2859.640644E-1121.4491251
Produced by FastQC (version 0.11.2)