FastQCFastQC Report
Sun 14 Apr 2019
SRR6575481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478980
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.13173827717232453No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5430.11336590254290367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9600.037.8204541
GTACATG18850.030.7681221
CAACGCA12550.028.8337385
TACATGG19600.028.7816182
ATCAACG12250.028.7726043
ACATGGG19500.028.1971513
TCAACGC12700.027.75314
AACGCAG13500.026.8046996
TATCAAC13950.026.2851452
CATGGGG9700.024.2243584
ACGCAGA15150.023.8853767
GTATTGG2453.237801E-1023.0952361
CGCAGAG15850.022.8305028
GTATAGC2103.713285E-822.45371
GTCTATG1703.3491942E-622.1895391
CTATAAG1503.204896E-522.0046251
ATAATCC1303.158083E-421.6901153
GTAGGAC1303.158083E-421.6901153
GTACTTG2901.1641532E-1021.137451
GGTACCT2701.1386874E-920.8867788
Produced by FastQC (version 0.11.2)