FastQCFastQC Report
Sun 14 Apr 2019
SRR6575494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406473
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC12240.3011270121262667No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10490.25807372199383477No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8670.21329830025610558No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7830.19263272099253825No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC7800.191894664590268No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6740.1658166717100521No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6590.16212638969870077No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6460.15892814528886298No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6430.1581900888865927No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6420.15794407008583594No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6300.1549918444767549No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6010.14785729925480903No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5980.1471192428525388No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC5520.13580237801772813No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5410.13309617120940384No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT4960.1220253251753499No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC4950.12177930637459315No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG4740.11661291155870132No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT4740.11661291155870132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.11120049794205274No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG4330.10652614072767441No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4200.10332789631783662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA754.1836756E-1156.3987467
ACGTTAA851.4188117E-1049.76368
GTATCAA9200.046.5855181
TCAAGAC1359.094947E-1238.2954443
GTGTTAC500.001700919637.5991636
GTACTAG2650.037.3225061
CAACGCA11000.037.17195
ATCAACG11200.036.5081183
TCTAGGG550.002715248834.1810573
CGTTAAC1256.2482286E-933.8392459
TATTCTC1554.1836756E-1133.3540955
ACGCAGA12950.031.5745857
ACGCAAG600.00415829931.3326388
CGCAGAG12750.031.3326388
TATCAAC13800.030.9920642
AACGCAG14200.030.4500276
AGTAGTA953.746944E-529.6835522
TTCTATA650.0061502228.9224362
GTACATG12950.028.731261
TCAACGC14750.028.3586924
Produced by FastQC (version 0.11.2)