FastQCFastQC Report
Sun 14 Apr 2019
SRR6575496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461989
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41010.887683472983123No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19660.42555125771392827No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18840.40780191736166876No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17060.3692728614750567No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12180.263642640842098No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12020.2601793549197059No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9590.207580699973376No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9440.20433386942113338No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC9400.20346804794053536No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9020.19524274387485416No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8070.17467948371065112No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA7370.1595276078001857No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT7240.15671368798824215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.1551985003971956No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7110.15389976817629858No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG6870.14870483929271044No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG6660.1441592765195708No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG6620.14329345503897278No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6510.14091244596732821No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6260.13550106171359058No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG6200.13420232949269353No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT6050.13095549894045097No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA6040.13073904357030144No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA6010.13008967745985295No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA5900.12770866838820838No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC5810.12576057005686284No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA5690.12316310561506875No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC5660.12251373950462024No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA5610.12143146265387271No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT5410.1171023552508826No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA5270.11407198006878952No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT5240.11342261395834101No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT5170.11190742636729446No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA5140.11125806025684595No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG5000.10822768507475287No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA4960.10736186359415485No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA4850.10498085452251028No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT4800.10389857767176276No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG4790.10368212230161325No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA4730.1023833900807162No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC4710.10195047934041719No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG4660.10086820248966966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3600.057.589081
GGACCGT250.005233609656.4012346
GTGCAAG3350.047.82161
AGGGTAA3150.044.7628866
AAGGGTA3150.044.7628865
GTATCAA15200.044.6384281
ATCAACG15800.042.241433
TCAACGC16150.041.9080354
GGTAATC3400.041.4670078
CAACGCA16250.041.3609055
TATCAAC16300.041.234032
AACGCAG17100.039.8546756
GTACATG25200.037.395511
GGGTAAT3800.037.1060757
ACATGGG25950.035.8620573
TACATGG26350.035.8527762
CATGGGG16650.035.003774
ACGCAGA21000.032.2292757
TGCAAGG4550.032.022682
ATGGGGG6900.032.0151985
Produced by FastQC (version 0.11.2)