FastQCFastQC Report
Sun 14 Apr 2019
SRR6575497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40600.9345170549362526No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21200.48797442277459496No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18320.4216835577938952No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16410.37771982442127844No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13000.2992295988712139No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12250.28196635278249No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10320.23754226618084057No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10280.2366215597227753No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC9830.22626361206954096No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8620.19841224171306643No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8050.1852921746856363No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7510.1728626375017551No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7350.169179811669494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.16066327693239024No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA6950.15997274708884127No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT6610.15214674219528646No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG6440.148233739748509No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA6410.1475432099049601No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC6290.14478109053076427No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG6060.13948702839688892No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA6000.13810596870979103No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5980.1376456154807584No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA5910.13603437917914415No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA5880.1353438493355952No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG5830.1341929662630136No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT5680.13074031704526884No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT5430.12498590168236087No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA5350.12314448876623033No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA5320.12245395892268138No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT5200.11969183954848556No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT5160.11877113309042028No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG5090.11715989678880605No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA5010.1153184838726755No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA4960.11416760080009392No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT4960.11416760080009392No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG4930.11347707095654495No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC4810.11071495158234913No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG4770.10979424512428386No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG4690.1079528322081533No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA4650.10703212575008804No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA4630.1065717725210554No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC4580.1054208894484738No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA4520.10403982976137591No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT4430.10196824023072903No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC4430.10196824023072903No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT4400.10127771038718007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2950.063.7472421
GGTAATC2850.049.4595078
GTATCAA13950.047.856131
GTGCAAG3750.045.1330451
ATCAACG14550.044.241623
TATCAAC15150.042.8242572
CAACGCA14950.042.7436035
TCAACGC14950.042.7436034
AACGCAG15950.040.3583376
AAGGGTA3500.040.2741745
GTAATCA4050.039.4454849
AGGGTAA3700.038.097196
ACTAGGC500.001703257637.5892263
GGGTAAT3950.035.6859747
GTACATG23150.034.5240171
ACGCAGA18950.033.7212077
CGCAGAG19150.033.3690268
CTTAGGT6400.033.0374073
TTAGGTA6450.032.78134
AGGTATA6600.032.748196
Produced by FastQC (version 0.11.2)