FastQCFastQC Report
Sun 14 Apr 2019
SRR6575508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473113
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7990.1688814300177759No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7340.15514264034173653No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7200.15218351641151268No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5690.12026725116409821No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5670.11984451917406624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.11350353932358655No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT5350.11308080733355456No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5220.1103330493983467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.053.3127481
ATCAACG13300.044.166233
CAACGCA13400.043.8366285
GTACTAG2250.041.8244821
TATCAAC15350.039.4923742
AACGCAG15500.039.4133766
TCAACGC15650.037.834514
GGTATCA4650.036.4277731
CGCAGAG16700.035.196628
ACGCAGA17300.033.954387
ATAGGTA2250.031.34845794
GCAGAGT18900.031.3484559
TAGGCAT3400.030.4071485
CTAGGCA3400.030.4071484
GTCTATA1401.8655555E-830.2480641
TATACAT953.751546E-529.6797054
ATACGGG2600.028.9186883
GTACATG20350.028.6708341
ACATGGG19800.028.4805243
TACATGG20050.028.3597852
Produced by FastQC (version 0.11.2)