FastQCFastQC Report
Sun 14 Apr 2019
SRR6575509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132729
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43040.37996731786685073No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24480.21611524027371065No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22070.19483918925003244No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16040.14160492050614049No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12090.1067333845959625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20600.052.7215081
GGTATCA5100.052.5469781
TCAACGC23250.045.0787244
ATCAACG23650.044.3162963
CAACGCA24300.043.3242875
AACGCAG25350.041.5297936
TATCAAC28250.037.945432
GTACATG43100.032.725561
ACGCAGA32300.032.4483077
CGCAGAG33400.031.8017988
TACATGG44550.031.4493472
ACATGGG44900.030.6698043
GTCTTAG8900.029.5829241
TCTTAGG9900.029.4441912
AGGTATA7750.029.109136
GCAGAGT36800.028.8635889
TAGGTAT8050.028.6081565
CTTAGGT7750.028.5026913
GTATAGT8650.027.710468
ACCTAAG14750.026.7750551
Produced by FastQC (version 0.11.2)