FastQCFastQC Report
Sun 14 Apr 2019
SRR6575509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132729
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44190.39011979034702915No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23750.2096706273080322No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21980.19404464792549675No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15740.1389564494243548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.056.363231
GGTATCA5100.049.8953171
TCAACGC23100.048.8318144
ATCAACG23350.048.5102773
CAACGCA24450.046.1355745
AACGCAG24650.045.570586
TATCAAC27150.041.7206232
TATAGGG952.116576E-839.5794642
ACGCAGA31050.036.023067
ACCTAAG14050.033.5397571
CGCAGAG33350.033.538718
GTACATG40900.032.2604871
GCAGAGT35600.031.5519
TACATGG42250.031.2595832
CTAAGAC16250.030.94813
TAAGACA18350.030.7360974
ACATGGG42800.030.308813
CCTAAGA17150.029.0499462
GTCTTAG9850.028.2261731
CTTAGGT8550.028.0354563
Produced by FastQC (version 0.11.2)