FastQCFastQC Report
Sun 14 Apr 2019
SRR6575510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037710
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42450.40907382602075726No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25650.24717888427402646No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22960.22125642038719873No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15860.15283653429185418No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11900.11467558373726763No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11020.10619537250291507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18000.051.7101021
GGTATCA4450.049.650151
TCAACGC19900.045.8059654
ATCAACG20350.044.3312723
CAACGCA21050.043.30355
AACGCAG22250.040.9680336
TATCAAC24300.037.143062
TTAGGTA7450.035.3187334
AGGTATA7250.034.9968646
CTTAGGT7600.034.003413
TACATGG38300.033.7533342
GTACATG38900.033.71611
TAGGTAT7700.032.9515955
GGTAATC4600.031.6648378
ACATGGG41000.031.5153583
ACGCAGA29600.030.6364887
GTCTTAG9700.030.531741
CGCAGAG30350.029.8794128
GGTATAG8700.029.1640557
TCTTAGG10350.029.0684812
Produced by FastQC (version 0.11.2)