FastQCFastQC Report
Sun 14 Apr 2019
SRR6575511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059642
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25080.23668371015871398No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15370.14504898824319912No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15350.14486024525264193No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14510.13693303964924003No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13160.12419288778662983No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11920.11249082237208416No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11130.10503547424507523No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10920.10305367284422476No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG10850.10239307237727459No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10850.10239307237727459No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10600.10003378499530972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.058.384491
GGTATCA4350.051.864491
TCAACGC23300.042.761914
ATCAACG23450.042.4883773
CAACGCA24100.041.1474155
AACGCAG24650.040.4199756
TATCAAC28900.035.1296652
GTACATG44850.033.2211721
TACATGG45550.032.710642
ACATGGG47200.031.4646033
ACGCAGA31700.031.430687
CGCAGAG34300.029.0481788
TAGGTAT5300.028.3760055
CATGGGG31100.028.2591044
AGGTATA5450.026.7326686
TTAGGTA5500.026.4896474
GCAGAGT37450.026.479389
TAAGACA13000.026.391054
AGAGTAC31750.026.2743410-11
CTTAGGT5450.025.8703253