FastQCFastQC Report
Sun 14 Apr 2019
SRR6575511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059642
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25550.24111917043680792No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14760.13929232703120487No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14120.13325255133337485No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12120.11437825227765604No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11740.11079213545706947No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11090.10465798826396085No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10900.10286492985366756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16000.054.7877271
GGTATCA5050.046.66261
TCAACGC20050.042.1987084
CAACGCA20900.040.4824945
ATCAACG21050.040.4192283
CTTAGGT5800.038.091793
AACGCAG22700.037.6865656
GTCTTAG7800.036.2532541
TTAGGTA6400.035.9879534
AGGTATA5900.035.8510256
GTACATG40050.035.185121
TACATGG40000.034.667582
TAGGTAT6400.034.5190545
TATCAAC25800.033.5242962
ACATGGG42200.032.8602683
GTATAGT6900.032.6942758
GGTATAG6550.032.2887157
TATAGTA7350.030.6925819
CATGGGG26400.030.4462094
ACGCAGA28550.029.9602077