FastQCFastQC Report
Sun 14 Apr 2019
SRR6575512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957513
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23480.2452186027761503No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14610.15258278477681245No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13330.1392148200598843No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12640.13200865157966524No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12630.13190421435531424No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11510.12020724522800214No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11160.11655194237571709No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10670.11143451838251804No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10140.10589934549191499No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9880.10318397765878896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15800.053.5379641
GGTATCA4800.046.994441
ATCAACG20850.040.3390243
TCAACGC21100.039.6383864
CAACGCA21750.038.453795
AACGCAG22100.038.2700166
TATCAAC25250.033.314872
TAGGTAT5300.031.0292025
TTAGGTA5150.031.0205944
ACGCAGA27250.030.8649047
GTACATG37650.030.705531
TACATGG37250.030.6567752
CTTAGGT5500.029.9008643
AGGTATA5400.029.5844556
CGCAGAG29250.028.5938438
ACATGGG40100.028.356293
GGTATAG5750.027.7836637
CATGGGG24500.027.425114
TATAGTA6400.027.1644049
AGAGTAC27400.026.15156710-11