FastQCFastQC Report
Sun 14 Apr 2019
SRR6575512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957513
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25260.2638084287106285No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14110.1473609235592624No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13390.1398414434059903No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11110.11602975625396209No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11040.11529869568350508No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10630.11101676948511403No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10370.10830140165198801No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10220.106734843286723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15450.056.935071
GGTATCA4650.045.522521
TCAACGC19000.044.764934
ATCAACG19350.043.4695363
CAACGCA19950.043.3398935
AACGCAG20900.041.8195726
TATCAAC24050.035.560612
ACGCAGA25550.033.8425067
GGGTATA2150.032.818561
ACCTAAG8350.032.674451
GTACATG34300.030.8571011
CGCAGAG27950.030.7909098
TACATGG34750.029.8848042
CTAAGAC9950.029.7529753
ACATGGG36800.028.6031023
AGGTATA5100.028.563046
GTATATG1652.935849E-928.509051
GTCTTAG6150.028.300451
GCAGAGT30650.028.2319329
TTAGGTA5000.028.1944874
Produced by FastQC (version 0.11.2)