FastQCFastQC Report
Sun 14 Apr 2019
SRR6575513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911566
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21180.23234741093897754No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16950.18594374954748202No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16420.18012957920764927No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15350.16839153720081707No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15060.16521019871298404No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13660.1498520129096522No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13250.14535425849581926No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12480.13690725630398676No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11950.13109308596415398No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11710.12846025411215425No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG11670.12802144880348762No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11120.12198787580932154No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10860.11913564130298848No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10520.11540579617932217No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10000.10970132716665605No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9940.10904311920365614No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT9880.10838491124065619No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9430.10344835151815665No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG9140.10026701303032365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4900.047.1037671
GTATCAA15400.047.1037641
TCAACGC17850.039.2306864
ATCAACG18300.038.009183
CAACGCA18850.037.149485
AACGCAG19600.035.7279476
GTACATG35950.031.8392621
TACATGG35950.031.5061972
CTTAAGT1501.0695658E-931.3318883
ACATGGG37800.030.3372253
ACCTAAG7000.029.6080821
ACGCAGA24600.028.2751227
TATCAAC25850.027.8169022
CTAAGAC8700.027.0102483
CGCAGAG26450.026.475158
GGTACCT7200.026.1099078
CATGGGG23050.026.098584
GGGTACC7450.025.864587
TGGGTAC7100.025.815716
TAAGACA11200.025.177414
Produced by FastQC (version 0.11.2)