FastQCFastQC Report
Sun 14 Apr 2019
SRR6575515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235234
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6280.26696821037775154No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4060.17259409779198587No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3240.13773519134138773No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3160.13433432241937815No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3070.13050834488211738No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2750.1169048691940791No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2640.11222867442631593No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2550.10840269688905517No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2450.10415161073654318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.1028762848907896No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2390.10160095904503601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT450.001010709241.7912031
GGCTCGC500.001701921537.5880979
ATGGGTA2150.037.1510245
CATGGGT3100.036.3755724
ACATGGG15650.034.826033
GTACATG15600.034.658571
GACAGTC550.002716852634.1709947
GTAGTAC550.002716852634.1709943
TACATGG16100.033.8742332
GTATCAA9000.032.388181
CATGGGG6250.031.5744
GGGTACC2400.031.323417
GAACCGA300.00416495631.3234182-83
TGGGTAC2450.030.6841586
GTGTAGC1859.094947E-1230.4962831
GGTACCT2700.029.5832258
GTATGTA650.006134712628.932371
GTAACTC650.006153869428.9139183
GTACCTG2850.028.0262139
CTTGTCG350.00884445926.84863910-11
Produced by FastQC (version 0.11.2)