FastQCFastQC Report
Sun 14 Apr 2019
SRR6575516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849394
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29160.34330357878675855No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16020.18860505254334267No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15440.1817766548857185No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12040.1417481168927494No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9820.11561183620322255No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8710.10254369585845909No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8570.10089546194110154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15600.059.3643531
TCAACGC19200.047.7326134
ATCAACG19650.046.4003223
CAACGCA20350.045.2661445
AACGCAG20700.044.500776
TATCAAC22200.041.5037352
GGTATCA2950.039.8384171
ACGCAGA25300.036.2239577
CGCAGAG26200.034.800248
GTACATG34600.033.1510931
GCAGAGT28000.032.8987859
TACATGG35500.032.443062
ACATGGG36700.030.9906833
ACCTAAG10750.030.610731
CTAAGAC11850.030.1423473
CCCTATA953.746055E-529.6901042
TAAGACA13500.028.895234
CATGGGG23900.028.7102364
AGGTATA5250.028.646566
AGAGTAC24900.028.12345910-11
Produced by FastQC (version 0.11.2)