FastQCFastQC Report
Sun 14 Apr 2019
SRR6575517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790269
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30950.39163879640983007No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16130.20410771522101967No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15840.20043807867953822No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12430.15728821451935987No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9450.11957953557586087No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8340.10553368536536294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15250.056.3943021
GGTATCA3450.053.125071
TCAACGC17750.045.7980274
ATCAACG17950.045.2877433
CAACGCA18400.043.9247865
AACGCAG19100.042.314986
ACCTAAG10350.039.0491941
CTAAGAC11250.038.8444943
TATCAAC22100.037.003132
TAAGACA13500.035.5030334
AGGTATA4600.034.7312286
TAAGGGT1107.7065124E-834.174044
ACGCAGA24050.033.9964187
CCTAAGA12200.033.515132
GTACATG34550.033.4611661
ACATGGG35050.032.711533
TAGGTAT4900.032.6048285
TACATGG35050.032.583652
CTTAGGT5200.032.531063
GTCTTAG5800.032.410521
Produced by FastQC (version 0.11.2)