FastQCFastQC Report
Sun 14 Apr 2019
SRR6575526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380009
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5140.13525995436950178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.12552334286819522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.051.4403571
ATCAACG12200.038.1385423
TCAACGC12450.037.3727074
CAACGCA12700.036.6370245
AACGCAG13000.036.5146186
TATCAAC13650.034.105152
TACATGG23300.032.2909972
GTACATG23350.031.8190781
ACATGGG23250.031.130533
ACGCAGA15550.030.2244457
CATGGGA11900.030.0161764
CGCAGAG15750.029.8406438
TGCCCGC650.00613388628.93771
TATAGTT1303.307032E-728.93772
ATAGTAC856.808471E-427.6464793
AACGCCG700.00882961826.8565795
GCAGAGT17950.026.706969
GTATTAT1101.01309255E-425.6493241
ATAACAC1501.1593202E-625.066143
ATGGGAG7250.023.9857035
Produced by FastQC (version 0.11.2)