FastQCFastQC Report
Sun 14 Apr 2019
SRR6575527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725489
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52440.722822813302476No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33470.4613440038374117No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30800.4245412404598829No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25110.3461113814268721No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16430.22646794093363234No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA15670.21599224798721967No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12750.1757435329825814No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12360.17036784844429068No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG12110.16692189681718123No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11020.15189754772298408No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10980.15134619546264658No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10530.1451434825338496No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10380.14307591155758392No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT10250.14128401671148702No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT10160.14004347412572762No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8630.11895425016781784No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8360.11523262241053965No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8330.11481910821528653No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8320.11468127015020214No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA7980.10999477593733331No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7940.10944342367699578No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT7770.10710017657056137No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA7550.10406773913870507No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC7310.10075962557668No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7300.10062178751159563No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA7260.10007043525125812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.049.4316671
GTATCAA19450.040.3620951
AAGGGTA5800.039.705915
GTGCAAG6200.039.4265251
TATCAAC20350.038.5770382
ATCAACG19850.038.1199533
GGTAATC6050.037.2883268
CAACGCA20350.037.183355
AGGGTAA6200.037.1442386
TCAACGC20900.036.4297144
GTACTAG1950.036.1604231
GTACATG36500.035.5461921
TACATGG37650.034.0858762
TGCAAGG6950.033.81912
ACATGGG38600.033.2401053
AACGCAG23450.033.0695426
ATTTAGG2000.032.9059831
GTAATCA7750.032.1411329
GGGCTAT2100.031.3325546
TTAACTC1351.3260433E-831.3325543
Produced by FastQC (version 0.11.2)