FastQCFastQC Report
Sun 14 Apr 2019
SRR6575528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706393
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52700.7460436329352074No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33850.47919500901056494No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33260.470842717863852No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25760.3646695253208908No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA16070.22749376055538492No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15870.22466247542090595No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12500.17695532090493535No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12200.1727083932032169No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11780.16676269442081107No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11410.161524816922025No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10940.15487129685599943No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT10640.15062436915428098No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10110.14312146354791172No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9870.13972392138653697No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9840.13929922861636512No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT9050.1281156523351732No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8900.12599218848431398No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8690.12301933909311105No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8510.12047118247208No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA8140.11523330497329391No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7910.11197732706864308No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7450.10546537125934147No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT7390.10461598571899779No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA7380.10447442146227384No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA7370.1043328572055499No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC7350.104049728692102No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA7280.10305877889503436No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA7220.10220939335469066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.10150157207107091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA4900.048.904375
GTGCAAG5450.043.9846341
GGTATCA4650.043.4653131
GTATCAA20450.041.8316841
ATCAACG19700.041.5008133
GGTAATC5700.041.216278
TATCAAC20650.040.51322
CAACGCA20500.039.8812685
AGGGTAA5900.038.226356
TCAACGC22000.037.162094
AACGCAG22850.036.602226
TTAGGTA11100.035.1340874
AGGTATA11200.034.820396
TAGGTAT11350.034.774195
CTTAGGT11500.034.320613
GTACTAG1651.8189894E-1234.1841351
TGCAAGG7050.033.9998972
GGTATAG11600.033.6196867
ACGCAGA24850.032.900047
GGGTAAT7150.032.8577277
Produced by FastQC (version 0.11.2)